However, the complementation analysis is completely bounded, peculiarly when chosen the polymerases that incorporate unusual substrates. For that Holliger and colleagues introduced a new technique, the compartmentalized particular-response trial (CSR), and have used this approach to alter the properties of a DNA polymerase (Ghadessy et al., 2001). In this process, a library of polymerase-encoding genes is cloned and expressed in Escherichia coli and the cells are encapsulated in a water-in-oil emulsion.

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